Job Title Lab Assistant-Media Preparation
Location(s)
Springfield, Missouri
Job Description Prepare broths, agars and reagents for Microbiology and Research departments; perform QC checks, data entry and cleaning tasks.
DUTIES AND RESPONSIBILITIES: DAILY
- Prepare media, reagents, and dilution water following SOP and or product label instructions, label made media and store at the correct temperature.
- Inspect made media to ensure that media visually is acceptable for use (i.e.-agar is dissolved, debris or particles are not present, tubes are filled, liquid media is not turbid, plates are completely poured, etc.). Perform Quality Check before sleeving plates and before stickering tube racks and record.
- Enter all QC information into LIMS and review for accuracy.
- Deliver media to appropriate labs in a timely manner
- Perform media pH's after autoclaving and record. Perform pH meter calibration and record result into calibration logbook.
- Ensure that positive and negative controls samples are provided to microbiology for all media.
- Complete all necessary QC records when Masterclave and AES dispensing equipment is used.
- Remove expired media from shelves and properly dispose of the containers.
- Deactivate media no longer available for use (not expired)
- Take a daily inventory of all media to be made utilizing minimum quantity guidelines.
- Take a weekly inventory of all media supplies and ensure that the Purchasing coordinator is provided an order list.
- Autoclave media at required parameters using proper PPE and fill in Autoclave Record Logbook.
- Perform volume checks on the Omnispense and record on appropriate form.
- Perform volume checks on media after autoclaving and record
- Clean media room counters and sanitize and mop media room floors
- Scan out inventory as supplies are used
- Store all media at the correct temperatures and ensure all labeling is complete and correct.
- Use scale per SOP to weigh powders for media preparation and clean scales thoroughly and sanitize.
- Perform daily weight check on media scale and record.
- Clean the microwaves as needed
- Monitor and document the DI water system daily and ensure that both lights are functioning. Notify management when lights are not on.
- Suggest improvements to LIMS concerning Standards and Reagents
- For all SOPs and forms pertaining to this job description, see LM 7011 Appendix B.
WEEKLY
- Clean and sanitize shelves where media is stored.
- Clean and sanitize shelves on walls.
- Check all media for certification/date received/date opened information.
- Ensure all media has received Media Certification prior to use and log Media Certifications when necessary.
- Properly label all containers once certified and place in appropriate labs
- Wipe out drawers, clean fronts of cabinets, and clean carts
- Clean and autoclave dispenser tubing.
- Complete Media Room Checklist
- Attend weekly meetings and contribute to improvement and quality efforts.
Requirements DAYS/HOURS:Tuesday - Saturday 1pm - 6pm
- Minimum of a High School diploma.
- Prefer current science major college student.
- Ability to multi-task
- Excellent communication skills
- Knowledge of cmolarity, normality, percent solutions and basic chemical math equations.
- Knowledge of aseptic technique
- Ability to work as a part of a work team, get along with co-workers and contribute in a positive manner.
